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James M. Fleckenstein, MD

Associate Professor of Medicine and Molecular Microbiology

Specialty Areas

Infectious Diseases

Board Certifications

National Board of Medical Examiners
American Board of Internal Medicine, Subspecialty Infectious Diseases

Areas of Clinical Interest

Infectious diseases, gastrointestinal infections

  • Location(s)
  • Education
  • Publication & Research

Location(s)

Location(s)

VA Medical Center: John Cochran Division

915 North Grand Boulevard
St. Louis, MO 63106

Office Phone: 314-652-4100
Fax: 314-289-6557

Education

Education

BS, Chemistry: Xavier University, Cincinnati, OH 1981
Residency, Internal Medicine: University of Michigan Hospitals, Ann Arbor, MI 1988
Chief Resident, Department of Medicine: University of Michigan Hospitals, Ann Arbor, MI 1989
Medical Degree: St. Louis University School of Medicine, St. Louis, MO 1985
Fellowship, Infectious Diseases: Walter Reed Army Medical Center, Washington, DC 1994
Fellowship, Advanced Bacterial Genetics: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 2001

Publication & Research

Publication & Research

Interrogation of a live-attenuated enterotoxigenic <i>Escherichia coli</i> vaccine highlights features unique to wild-type infection.
Chakraborty S, Randall A, Vickers TJ, Molina D, Harro CD, DeNearing B, Brubaker J, Sack DA, Bourgeois AL, Felgner PL, Liang X, Mani S, Wenzel H, Townsend RR, Gilmore PE, Darsley MJ, Rasko DA, Fleckenstein JM
NPJ Vaccines. 2019; 437. doi: 10.1038/s41541-019-0131-7.

PMID:
    31482013
    [PubMed]
Related citations


Enterotoxigenic Escherichia coli-blood group A interactions intensify diarrheal severity.
Kumar P, Kuhlmann FM, Chakraborty S, Bourgeois AL, Foulke-Abel J, Tumala B, Vickers TJ, Sack DA, DeNearing B, Harro CD, Wright WS, Gildersleeve JC, Ciorba MA, Santhanam S, Porter CK, Gutierrez RL, Prouty MG, Riddle MS, Polino A, Sheikh A, Donowitz M, Fleckenstein JM
J Clin Invest. 2019 Jul 1; 129(7)2980. doi: 10.1172/JCI130874.

PMID:
    31259744
    [PubMed]
Related citations


Enterotoxigenic Escherichia coli Infections.
Fleckenstein JM, Kuhlmann FM
Curr Infect Dis Rep. 2019 Mar 4; 21(3)9. doi: 10.1007/s11908-019-0665-x.

PMID:
    30830466
    [PubMed]
Related citations


Molecular Determinants of Enterotoxigenic Escherichia coli Heat-Stable Toxin Secretion and Delivery.
Zhu Y, Luo Q, Davis SM, Westra C, Vickers TJ, Fleckenstein JM
Infect Immun. 2018 Nov; 86(11)pii: e00526-18. doi: 10.1128/IAI.00526-18.

PMID:
    30126899
    [PubMed - indexed for MEDLINE]
Related citations


Human Experimental Challenge With Enterotoxigenic Escherichia coli Elicits Immune Responses to Canonical and Novel Antigens Relevant to Vaccine Development.
Chakraborty S, Randall A, Vickers TJ, Molina D, Harro CD, DeNearing B, Brubaker J, Sack DA, Bourgeois AL, Felgner PL, Liang X, Mani S, Wenzel H, Townsend RR, Gilmore PE, Darsley MJ, Rasko DA, Fleckenstein JM
J Infect Dis. 2018 Sep 22; 218(9)1436-1446. doi: 10.1093/infdis/jiy312.

PMID:
    29800314
    [PubMed - indexed for MEDLINE]
Related citations


Enterotoxigenic Escherichia coli-blood group A interactions intensify diarrheal severity.
Kumar P, Kuhlmann FM, Chakraborty S, Bourgeois AL, Foulke-Abel J, Tumala B, Vickers TJ, Sack DA, DeNearing B, Harro CD, Wright WS, Gildersleeve JC, Ciorba MA, Santhanam S, Porter CK, Gutierrez RL, Prouty MG, Riddle MS, Polino A, Sheikh A, Donowitz M, Fleckenstein JM
J Clin Invest. 2018 Aug 1; 128(8)3298-3311. doi: 10.1172/JCI97659.

PMID:
    29771685
    [PubMed - indexed for MEDLINE]
Related citations


A Role for Salivary Peptides in the Innate Defense Against Enterotoxigenic Escherichia coli.
Brown JW, Badahdah A, Iticovici M, Vickers TJ, Alvarado DM, Helmerhorst EJ, Oppenheim FG, Mills JC, Ciorba MA, Fleckenstein JM, Bullitt E
J Infect Dis. 2018 Apr 11; 217(9)1435-1441. doi: 10.1093/infdis/jiy032.

PMID:
    29528423
    [PubMed - indexed for MEDLINE]
Related citations


Comparative genomics and transcriptomics of Escherichia coli isolates carrying virulence factors of both enteropathogenic and enterotoxigenic E. coli.
Hazen TH, Michalski J, Luo Q, Shetty AC, Daugherty SC, Fleckenstein JM, Rasko DA
Sci Rep. 2017 Jun 14; 7(1)3513. doi: 10.1038/s41598-017-03489-z.

PMID:
    28615618
    [PubMed - indexed for MEDLINE]
Related citations


Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology.
Sahl JW, Sistrunk JR, Baby NI, Begum Y, Luo Q, Sheikh A, Qadri F, Fleckenstein JM, Rasko DA
Sci Rep. 2017 Jun 13; 7(1)3402. doi: 10.1038/s41598-017-03631-x.

PMID:
    28611468
    [PubMed - indexed for MEDLINE]
Related citations


Providing Structure to Enterotoxigenic Escherichia coli Vaccine Development.
Fleckenstein JM
J Infect Dis. 2017 Jul 1; 216(1)1-3. doi: 10.1093/infdis/jix146.

PMID:
    28541516
    [PubMed - indexed for MEDLINE]
Related citations


Highly conserved type 1 pili promote enterotoxigenic E. coli pathogen-host interactions.
Sheikh A, Rashu R, Begum YA, Kuhlman FM, Ciorba MA, Hultgren SJ, Qadri F, Fleckenstein JM
PLoS Negl Trop Dis. 2017 May; 11(5)e0005586. doi: 10.1371/journal.pntd.0005586.

PMID:
    28531220
    [PubMed - indexed for MEDLINE]
Related citations


Dynamic Interactions of a Conserved Enterotoxigenic Escherichia coli Adhesin with Intestinal Mucins Govern Epithelium Engagement and Toxin Delivery.
Kumar P, Kuhlmann FM, Bhullar K, Yang H, Vallance BA, Xia L, Luo Q, Fleckenstein JM
Infect Immun. 2016 Dec; 84(12)3608-3617.

PMID:
    27736776
    [PubMed - indexed for MEDLINE]
Related citations


Immunogenicity and Protective Efficacy against Enterotoxigenic Escherichia coli Colonization following Intradermal, Sublingual, or Oral Vaccination with EtpA Adhesin.
Luo Q, Vickers TJ, Fleckenstein JM
Clin Vaccine Immunol. 2016 Jul; 23(7)628-37. doi: 10.1128/CVI.00248-16.

PMID:
    27226279
    [PubMed - indexed for MEDLINE]
Related citations


Blood Group O-Dependent Cellular Responses to Cholera Toxin: Parallel Clinical and Epidemiological Links to Severe Cholera.
Kuhlmann FM, Santhanam S, Kumar P, Luo Q, Ciorba MA, Fleckenstein JM
Am J Trop Med Hyg. 2016 Aug 3; 95(2)440-3. doi: 10.4269/ajtmh.16-0161.

PMID:
    27162272
    [PubMed - indexed for MEDLINE]
Related citations


Overcoming Enterotoxigenic Escherichia coli Pathogen Diversity: Translational Molecular Approaches to Inform Vaccine Design.
Fleckenstein JM, Rasko DA
Methods Mol Biol. 2016; 1403363-83. doi: 10.1007/978-1-4939-3387-7_19.

PMID:
    27076141
    [PubMed - indexed for MEDLINE]
Related citations


Examination of the Enterotoxigenic Escherichia coli Population Structure during Human Infection.
Sahl JW, Sistrunk JR, Fraser CM, Hine E, Baby N, Begum Y, Luo Q, Sheikh A, Qadri F, Fleckenstein JM, Rasko DA
MBio. 2015 Jun 9; 6(3)e00501. doi: 10.1128/mBio.00501-15.

PMID:
    26060273
    [PubMed - indexed for MEDLINE]
Related citations


Conservation and immunogenicity of novel antigens in diverse isolates of enterotoxigenic Escherichia coli.
Luo Q, Qadri F, Kansal R, Rasko DA, Sheikh A, Fleckenstein JM
PLoS Negl Trop Dis. 2015 Jan; 9(1)e0003446. doi: 10.1371/journal.pntd.0003446.

PMID:
    25629897
    [PubMed - indexed for MEDLINE]
Related citations


Contribution of the highly conserved EaeH surface protein to enterotoxigenic Escherichia coli pathogenesis.
Sheikh A, Luo Q, Roy K, Shabaan S, Kumar P, Qadri F, Fleckenstein JM
Infect Immun. 2014 Sep; 82(9)3657-66. doi: 10.1128/IAI.01890-14.

PMID:
    24935979
    [PubMed - indexed for MEDLINE]
Related citations


Designing vaccines to neutralize effective toxin delivery by enterotoxigenic Escherichia coli.
Fleckenstein JM, Sheikh A
Toxins (Basel). 2014 Jun 10; 6(6)1799-812. doi: 10.3390/toxins6061799.

PMID:
    24918359
    [PubMed - indexed for MEDLINE]
Related citations


Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.
Luo Q, Kumar P, Vickers TJ, Sheikh A, Lewis WG, Rasko DA, Sistrunk J, Fleckenstein JM
Infect Immun. 2014 Feb; 82(2)509-21. doi: 10.1128/IAI.01106-13.

PMID:
    24478067
    [PubMed - indexed for MEDLINE]
Related citations

Areas of Research Interest

enterotoxigenic Escherichia coli (ETEC) molecular pathogenesis and vaccine development